Posts from 2015-04-15

Exposición materna a la aflatoxina durante los primeros estadios del embarazo se asocia con cambios en la metilación del ADN en niños
Int J Epidemiol. 2015 Apr 7. pii: dyv027. [Epub ahead of print]

Exposure to aflatoxin B1 in utero is associated with DNA methylation in white blood cells of infants in The Gambia.

Author information

  • 1Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK.
  • 2Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK.
  • 3Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK medmnr@leeds.ac.uk.

Abstract

BACKGROUND:

Exposure to environmental toxins during embryonic development may lead to epigenetic changes that influence disease risk in later life. Aflatoxin is a contaminant of staple foods in sub-Saharan Africa, is a known human liver carcinogen and has been associated with stunting in infants.

METHODS:

We have measured aflatoxin exposure in 115 pregnant women in The Gambia and examined the DNA methylation status of white blood cells from their infants at 2-8 months old (mean 3.6 ± 0.9). Aflatoxin exposure in women was assessed using an ELISA method to measure aflatoxin albumin (AF-alb) adducts in plasma taken at 1-16 weeks of pregnancy. Genome-wide DNA methylation of infant white blood cells was measured using the Illumina Infinium HumanMethylation450beadchip.

RESULTS:

AF-alb levels ranged from 3.9 to 458.4 pg/mg albumin. We found that aflatoxin exposure in the mothers was associated to DNA methylation in their infants for 71 CpG sites (false discovery rate  0.05), with an average effect size of 1.7% change in methylation. Aflatoxin-associated differential methylation was observed in growth factor genes such as FGF12 and IGF1, and immune-relatedgenes such as CCL28, TLR2 and TGFBI. Moreover, one aflatoxin-associated methylation region (corresponding to the miR-4520b locus) was identified.

CONCLUSIONS:

This study shows that maternal exposure to aflatoxin during the early stages of pregnancy is associated with differential DNA methylation patterns of infants, including in genes related to growth and immune function. This reinforces the need for interventions to reduce aflatoxin exposure, especially during critical periods of fetal and infant development.

© The Author 2015; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association.

KEYWORDS:

Aflatoxin; DNA methylation; in utero exposure

PMID:
 
25855716
 
[PubMed - as supplied by publisher]
Maternal exposure to aflatoxin during the early stages of pregnancy is associated with differential DNA methylation patterns of infants.
Int J Epidemiol. 2015 Apr 7. pii: dyv027. [Epub ahead of print]

Exposure to aflatoxin B1 in utero is associated with DNA methylation in white blood cells of infants in The Gambia.

Author information

  • 1Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK.
  • 2Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK.
  • 3Epigenetics Group, International Agency for Research on Cancer (IARC), Lyon, France, LICAMM, School of Medicine, University of Leeds, Leeds, UK, MRC International Nutrition Group at LSHTM, UK & MRC Keneba, MRC Unit, The Gambia, Genetic Cancer Susceptibility Group, IARC, Lyon, France, Director, IARC, Lyon, France, MRC Human Nutrition Research, Cambridge, UK and UK Institute of Global Food Security, Queen's University Belfast, Belfast, UK medmnr@leeds.ac.uk.

Abstract

BACKGROUND:

Exposure to environmental toxins during embryonic development may lead to epigenetic changes that influence disease risk in later life. Aflatoxin is a contaminant of staple foods in sub-Saharan Africa, is a known human liver carcinogen and has been associated with stunting in infants.

METHODS:

We have measured aflatoxin exposure in 115 pregnant women in The Gambia and examined the DNA methylation status of white blood cells from their infants at 2-8 months old (mean 3.6 ± 0.9). Aflatoxin exposure in women was assessed using an ELISA method to measure aflatoxin albumin (AF-alb) adducts in plasma taken at 1-16 weeks of pregnancy. Genome-wide DNA methylation of infant white blood cells was measured using the Illumina Infinium HumanMethylation450beadchip.

RESULTS:

AF-alb levels ranged from 3.9 to 458.4 pg/mg albumin. We found that aflatoxin exposure in the mothers was associated to DNA methylation in their infants for 71 CpG sites (false discovery rate  0.05), with an average effect size of 1.7% change in methylation. Aflatoxin-associated differential methylation was observed in growth factor genes such as FGF12 and IGF1, and immune-relatedgenes such as CCL28, TLR2 and TGFBI. Moreover, one aflatoxin-associated methylation region (corresponding to the miR-4520b locus) was identified.

CONCLUSIONS:

This study shows that maternal exposure to aflatoxin during the early stages of pregnancy is associated with differential DNA methylation patterns of infants, including in genes related to growth and immune function. This reinforces the need for interventions to reduce aflatoxin exposure, especially during critical periods of fetal and infant development.

© The Author 2015; all rights reserved. Published by Oxford University Press on behalf of the International Epidemiological Association.

KEYWORDS:

Aflatoxin; DNA methylation; in utero exposure

PMID:
 
25855716
 
[PubMed - as supplied by publisher]
Primera evidencia mostrando que el ejercicio reprograma el metiloma en esperma.
Epigenomics. 2015 Apr 13:1-15. [Epub ahead of print]

Genome-wide sperm DNA methylation changes after 3 months of exercise training in humans.

Author information

  • 1Faculty of Science & Technology, Federation University Australia, Y Building, University Drive, Mt Helen, Victoria, Australia 3350.

Abstract

AIM:

DNA methylation programs gene expression and is involved in numerous biological processes. Accumulating evidence supports transgenerational inheritance of DNA methylation changes in mammals via germ cells. Our aim was to determine the effect of exercise on sperm DNA methylation.

MATERIALS & METHODS:

Twenty-four men were recruited and assigned to an exercise intervention or control group. Clinical parameters were measured and sperm samples were donated by subjects before and after the 3-month time-period. Mature sperm global and genome-wide DNA methylation was assessed using an ELISA assay and the 450K BeadChip (Illumina).

RESULTS:

Global and genome-wide sperm DNA methylation was altered after 3 months of exercise training. DNA methylation changes occurred in genes related to numerous diseases such as Schizophrenia and Parkinson's disease.

CONCLUSIONS:

Our study provides the first evidence showing exercise training reprograms the sperm methylome. Whether these DNA methylation changes are inherited to future generations warrants attention.

KEYWORDS:

disease risk; epigenetics; sperm; transgenerational inheritance

PMID:
 
25864559
 
[PubMed - as supplied by publisher]
First evidence showing exercise training reprograms the sperm methylome.
Epigenomics. 2015 Apr 13:1-15. [Epub ahead of print]

Genome-wide sperm DNA methylation changes after 3 months of exercise training in humans.

Author information

  • 1Faculty of Science & Technology, Federation University Australia, Y Building, University Drive, Mt Helen, Victoria, Australia 3350.

Abstract

AIM:

DNA methylation programs gene expression and is involved in numerous biological processes. Accumulating evidence supports transgenerational inheritance of DNA methylation changes in mammals via germ cells. Our aim was to determine the effect of exercise on sperm DNA methylation.

MATERIALS & METHODS:

Twenty-four men were recruited and assigned to an exercise intervention or control group. Clinical parameters were measured and sperm samples were donated by subjects before and after the 3-month time-period. Mature sperm global and genome-wide DNA methylation was assessed using an ELISA assay and the 450K BeadChip (Illumina).

RESULTS:

Global and genome-wide sperm DNA methylation was altered after 3 months of exercise training. DNA methylation changes occurred in genes related to numerous diseases such as Schizophrenia and Parkinson's disease.

CONCLUSIONS:

Our study provides the first evidence showing exercise training reprograms the sperm methylome. Whether these DNA methylation changes are inherited to future generations warrants attention.

KEYWORDS:

disease risk; epigenetics; sperm; transgenerational inheritance

PMID:
 
25864559
 
[PubMed - as supplied by publisher]

 

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